Dasatinib Ointment Promotes Healing of Murine Excisional Skin Wound

Dasatinib, a tyrosine kinase inhibitor, has been shown to produce anti-inflammatory activity and impair vascular integrity in vivo, including during skin wound healing, potentially promoting the repair process. Given that dasatinib is lipophilic small molecule capable of penetrating skin, topical might provide benefits healing. In present study, we investigated impact ointments healing mice. A full thickness excisional (4 mm diameter) was generated on shaved dorsum eight-week-old C57BL/6 Dasatinib ointment (0.1 or 0.2% w/w) base applied twice daily (every 12 h) for 10 days. Elizabethan collars were used prevent animal licking. The size monitored 14 results showed ointments, particularly 0.1% dasatinib, promoted 16–23% reduction (p < 0.05) day 2 6 postinjury compared controls. Immunohistochemistry analyses demonstrated neutrophils (38% reduction, p = 0.04), macrophages (47% 0.005), tumor necrosis factor-α levels (73% 0.01), together with an induction leakage-mediated fibrin(ogen) accumulation (2.5-fold increase, 0.01) 3 (an early phase repair) dasatinib-treated mice relative control hyperpermeability activities associated enhanced process, increased keratinocyte proliferation (1.8-fold increase Ki67+ cells, augmented angiogenesis (1.7-fold CD31+ area, 0.05), base-treated group. Following treatment ointment, minor bleeding scab reformation observed late phase, which contributed delayed conclusion, our data suggest mainly at 0.1%, promotes process by reducing inflammation producing local temporal leakage, leading deposition, re-epithelialization, angiogenesis. Therefore, be potential novel candidate facilitate